论文标题
使用彩色空间光干扰显微镜（CSLIM）量化脑组织中的髓磷脂
Quantifying myelin in brain tissue using color spatial light interference microscopy (cSLIM)
论文作者
论文摘要
大脑缺乏髓鞘性与神经发育延迟有关，尤其是在高风险婴儿中，例如与胎龄相对于胎龄（SGA）的小小的。需要新的方法来进一步研究这种情况。在这里，我们采用彩色空间光干扰显微镜（CSLIM），该显微镜使用Brightfield物镜和RGB摄像头与常规的Brightfield图像结合使用，以纳米级灵敏度生成带有纳米级灵敏度的路径映射。使用用Luxol Fast Blue染色的组织切片，将髓磷脂结构从Brightfield图像中进行了细分。使用二进制掩码，在相应的相图中定量分析了这些部分。我们首先使用清晰度方法去除组织脂质并验证CSLIM对脂质含量的敏感性。然后，我们将CSLIM应用于脑组织学切片。这些标本来自先前的MRI研究，这表明适合妊娠年龄（AGA）小猪的内部囊髓质（ICM）与胎龄（SGA）小猪相比，内部囊髓质（ICM）增加，并且水解脂肪饮食在两者中都改善了ICM。样品的身份被视而不见，直到统计分析后。
Deficient myelination of the brain is associated with neurodevelopmental delays, particularly in high-risk infants, such as those born small in relation to their gestational age (SGA). New methods are needed to further study this condition. Here, we employ Color Spatial Light Interference Microscopy (cSLIM), which uses a brightfield objective and RGB camera to generate pathlength-maps with nanoscale sensitivity in conjunction with a regular brightfield image. Using tissue sections stained with Luxol Fast Blue, the myelin structures were segmented from a brightfield image. Using a binary mask, those portions were quantitatively analyzed in the corresponding phase maps. We first used the CLARITY method to remove tissue lipids and validate the sensitivity of cSLIM to lipid content. We then applied cSLIM to brain histology slices. These specimens are from a previous MRI study, which demonstrated that appropriate for gestational age (AGA) piglets have increased internal capsule myelination (ICM) compared to small for gestational age (SGA) piglets and that a hydrolyzed fat diet improved ICM in both. The identity of samples was blinded until after statistical analyses.
