论文标题
大鼠坐骨神经中诱发神经活动的光学相干断层扫描成像
Optical coherence tomography imaging of evoked neural activity in sciatic nerve of rat
论文作者
论文摘要
意义：使用光学相干断层扫描（OCT）在髓鞘组织中成像神经活动为外围和中枢神经系统中功能成像创造了新的可能性。目的：研究OCT图像的变化，以响应大鼠体外大鼠坐骨神经的诱发神经活性。方法：使用扫描的源极化敏感的OCT系统在大鼠的周围神经上获得M扫描，而神经袖口获得了获得的电神经记录。从总共10个受试者中：3个没有刺激（对照），有3个具有爪子刺激，而4个具有神经刺激。计算了针对溶液中镜子和微球的每个受试者的OCT信号强度，相位障碍，相位和频谱的变化。结果：观察到的3个PAW刺激和2个神经刺激受试者的强度变化以及2个PAW刺激受试者中的频谱振幅的变化高于参考噪声水平，并且与神经活动期间离子电流的渗透肿胀在时间上是一致的。结论：以前在鱿鱼和蟹神经中表征的渗透肿胀产生的光散射变化也被认为是在骨髓纤维中发生的，该尺度可用于使用OCT检测到
Significance: Imaging neural activity in myelinated tissue using optical coherence tomography (OCT) creates new possibilities for functional imaging in the peripheral and central nervous systems. Aim: To investigate changes in OCT images in response to evoked neural activity in sciatic nerve of rat in vitro. Approach: M-scans were obtained on peripheral nerves of rat using a swept source polarisation sensitive OCT system, while a nerve cuff acquired electrical neural recordings. From a total of 10 subjects: 3 had no stimulation (controls), 3 had paw stimulation, and 4 had nerve stimulation. Changes in the OCT signal intensity, phase retardation, phase, and frequency spectra were calculated for each subject and reference samples of a mirror and microspheres in solution. Results: Observed changes in intensity in 3 paw stimulation and 2 nerve stimulation subjects and changes in frequency spectra amplitude in 2 paw stimulation subjects were above the reference noise level and were temporally consistent with osmotic swelling from ion currents during neural activity. Conclusion: Light scattering changes produced by osmotic swelling, which have previously been characterised in squid and crab nerve, are also thought to occur in myelinated fibres on a scale which is detectable using OCT
