论文标题
分组或时间门控的周期性衰减的连续和离散的相位分析
Continuous and discrete phasor analysis of binned or time-gated periodic decays
论文作者
论文摘要
在存在时间门控或货架的情况下,通过相分子方法对定期激发的发光衰减进行定期激发的发光衰减的时间分析。得出了平方门离散配置的分析表达式,并将这种修饰的周期性单指数衰减的相位者的基因座与规范的单次半圆进行了比较。还讨论了IRF偏移,衰减截断和栅极形状的影响。最后,对于某些简单的情况,对阶段和模量生命的修改表达式进行了研究。介绍了关于修改后的量子校准方法的讨论,并以文献中的例子结束了新概念的例证。
Time-resolved analysis of periodically excited luminescence decays by the phasor method in the presence of time-gating or binning is revisited. Analytical expressions for discrete configurations of square gates are derived and the locus of the phasors of such modified periodic single-exponential decays is compared to the canonical uni-versal semicircle. The effects of IRF offset, decay truncation and gate shape are also discussed. Finally, modified expressions for the phase and modulus lifetimes are pro-vided for some simple cases. A discussion of a modified phasor calibration approach is presented, and illustration of the new concepts with examples from the literature conclude this work.