论文标题
通过角度倾斜的，波长 - 多头ptychographic调制
Super-resolved multispectral lensless microscopy via angle-tilted, wavelength-multiplexed ptychographic modulation
论文作者
论文摘要
我们向多光谱无镜头的片上显微镜报告了一种角度倾斜，波长 - 多形的ptychographic调制方法。在这种方法中，我们同时用5个波长照明样品。在光谱分散的照明路径上添加了棱镜。因此，不同波长处的光线以略有不同的入射角击中样品，从而打破了混合状态ptychographic重建的歧义。在检测路径上，我们在样品和单色图像传感器之间放置一个薄扩散器，以将光谱信息编码为2D强度测量。通过将样品扫描到不同的X-Y位置，我们获得了一系列单色图像，用于重建5个波长的5个复杂对象曲线。将上采样过程集成到恢复过程中，以绕过成像器像素大小施加的分辨率限制。我们使用具有1.85微米像素尺寸的图像传感器来证明0.55微米的半诉分辨率。我们还展示了用于数字病理应用的染色组织切片的定量和高质量的多光谱重建。
We report an angle-tilted, wavelength-multiplexed ptychographic modulation approach for multispectral lensless on-chip microscopy. In this approach, we illuminate the specimen with lights at 5 wavelengths simultaneously. A prism is added at the illumination path for spectral dispersion. Lightwaves at different wavelengths, thus, hit the specimen at slightly different incident angles, breaking the ambiguities in mixed state ptychographic reconstruction. At the detection path, we place a thin diffuser in-between the specimen and the monochromatic image sensor for encoding the spectral information into 2D intensity measurements. By scanning the sample to different x-y positions, we acquire a sequence of monochromatic images for reconstructing the 5 complex object profiles at the 5 wavelengths. An up-sampling procedure is integrated into the recovery process to bypass the resolution limit imposed by the imager pixel size. We demonstrate a half-pitch resolution of 0.55 microns using an image sensor with 1.85-micron pixel size. We also demonstrate quantitative and high-quality multispectral reconstructions of stained tissue sections for digital pathology applications.
