论文标题
两光片光显微镜的斑马鱼幼虫中癫痫发作的快速全脑成像
Fast whole-brain imaging of seizures in zebrafish larvae by two-photon light-sheet microscopy
论文作者
论文摘要
灯泡荧光显微镜（LSFM）使斑马鱼幼虫的实时全脑功能成像。但是，传统的一个光子LSFM可能会由于使用可见的激发光而引起不良的视觉刺激。使用近红外看不见的光的两光子（2p）激发的使用提供了对神经元电路动力学的无偏研究。但是，由于2p吸收过程的效率低，该技术的成像速度通常受到信噪比的比例的限制。在这里，我们描述了专为非侵入性成像设计的2P LSFM设置，该设置可以使幼虫斑马鱼脑（5 Hz）的Quintuplic Quintuprictulic Quintuprictulicplictupricplicstulclicplicstuprictulcimentrics的最先进的体积采集率（5 Hz）。我们将系统应用于药理学诱导的急性癫痫发作的研究，表征了病理活性的时空动力学，并首次描述了尾尾式发作波（CRIWS）。
Light-sheet fluorescence microscopy (LSFM) enables real-time whole-brain functional imaging in zebrafish larvae. Conventional one photon LSFM can however induce undesirable visual stimulation due to the use of visible excitation light. The use of two-photon (2P) excitation, employing near-infrared invisible light, provides unbiased investigation of neuronal circuit dynamics. However, due to the low efficiency of the 2P absorption process, the imaging speed of this technique is typically limited by the signal-to-noise-ratio. Here, we describe a 2P LSFM setup designed for non-invasive imaging that enables quintuplicating state-of-the-art volumetric acquisition rate of the larval zebrafish brain (5 Hz) while keeping low the laser intensity on the specimen. We applied our system to the study of pharmacologically-induced acute seizures, characterizing the spatial-temporal dynamics of pathological activity and describing for the first time the appearance of caudo-rostral ictal waves (CRIWs).
