论文标题
荧光寿命成像显微镜（FLIM）的反向卷积
Deconvolution in Fluorescence Lifetime imaging microscopy (FLIM)
论文作者
论文摘要
荧光寿命成像显微镜（FLIM）是了解细胞和组织中化学微环境的重要技术，因为与常规荧光成像相比，它提供了额外的对比度。当激发衍射极限内的两个荧光团时，产生的发射会导致非线性空间失真和强度（幅度）和寿命（相）成分的定位效应。为了解决这个问题，在这项工作中，我们为FLIM中的卷积提供了一个理论模型，以描述所产生的行为与常规荧光显微镜的不同。 We then present a Richardson-Lucy (RL) based deconvolution including total variation (TV) regularization method to correct for the distortions in FLIM measurements due to optical convolution, and experimentally demonstrate this FLIM deconvolution method on a multi-photon microscopy (MPM)-FLIM images of fluorescent-labeled fixed bovine pulmonary arterial endothelial (BPAE) cells.
Fluorescence lifetime imaging microscopy (FLIM) is an important technique to understand the chemical micro-environment in cells and tissues since it provides additional contrast compared to conventional fluorescence imaging. When two fluorophores within a diffraction limit are excited, the resulting emission leads to non-linear spatial distortion and localization effects in intensity (magnitude) and lifetime (phase) components. To address this issue, in this work, we provide a theoretical model for convolution in FLIM to describe how the resulting behavior differs from conventional fluorescence microscopy. We then present a Richardson-Lucy (RL) based deconvolution including total variation (TV) regularization method to correct for the distortions in FLIM measurements due to optical convolution, and experimentally demonstrate this FLIM deconvolution method on a multi-photon microscopy (MPM)-FLIM images of fluorescent-labeled fixed bovine pulmonary arterial endothelial (BPAE) cells.
