论文标题
在相邻化学相同的荧光团中对非加性作用进行建模
Modeling Non-additive Effects in Neighboring Chemically Identical Fluorophores
论文作者
论文摘要
定量荧光分析通常用于得出生物分子复合物的化学特性，包括石化的化学特性。 One fundamental underlying assumption in the analysis of fluorescence data -- whether it be the determination of protein complex stoichiometry by super-resolution, or step-counting by photobleaching, or the determination of RNA counts in diffraction-limited spots in RNA fluorescence {\it in situ} hybridization (RNA-FISH) experiments -- is that fluorophores behave identically and do not interact.然而，最近对荧光团标记的DNA-鸟类结构（例如荧光灯摄起群）进行的实验揭示了相同的荧光团之间相互作用的性质，因为这些结构将它们更加靠近，从而提出了关于荧光酚不相互作用的建模假设的有效性的问题。在这里，我们分析了来自荧光纤维的脉冲照明下的光子到达数据，其中染料之间的距离范围为2-10 nm。我们讨论了亮度对定量荧光分析的非促进性的含义。
Quantitative fluorescence analysis is often used to derive chemical properties, including stoichiometries, of biomolecular complexes. One fundamental underlying assumption in the analysis of fluorescence data -- whether it be the determination of protein complex stoichiometry by super-resolution, or step-counting by photobleaching, or the determination of RNA counts in diffraction-limited spots in RNA fluorescence {\it in situ} hybridization (RNA-FISH) experiments -- is that fluorophores behave identically and do not interact. However, recent experiments on fluorophore-labeled DNA-origami structures such as fluorocubes have shed light on the nature of the interactions between identical fluorophores as these are brought closer together, thereby raising questions on the validity of the modeling assumption that fluorophores do not interact. Here, we analyze photon arrival data under pulsed illumination from fluorocubes where distances between dyes range from 2-10 nm. We discuss the implications of non-additivity of brightness on quantitative fluorescence analysis.
