论文标题
基于菲涅耳症的数字全息显微镜中的结构化照明
Structured illumination in Fresnel biprism-based digital holographic microscopy
论文作者
论文摘要
结构化照明（SI）结构可以在数字全息（DH）显微镜中采用以增强空间分辨率。在本文中，我们提出并演示了DH显微镜的自我引用的共同路径构型中的紧凑而简单的SI方法。由紧凑型磁盘和由菲涅尔双二元主义形成的SI模式的组合引入了一个低成本且高度稳定的系统，以对幅度和相位对象进行成像，并具有双重改进的空间分辨率。标准测试样品的实验结果与常规成像仪相比验证了预测的分辨率增强。与传统的DH显微镜相比，提出的DH方法允许成像具有明显清晰度的活细胞。
The structured illumination (SI) architecture can be adopted in a digital holographic (DH) microscope to enhance the spatial resolution. In this paper, we propose and demonstrate a compact and simple SI method in a self-referencing common-path configuration of DH microscopy. The combination of SI pattern generated by a compact disk and common-path off-axis geometry formed by Fresnel biprism introduces a low-cost and highly stable system to image both amplitude and phase objects with a twofold improvement of the spatial resolution. Experimental results for the case of a standard test sample validate the predicted resolution enhancement compared to that of the conventional imager. The presented DH method allows for imaging living cells with strikingly improved clarity compared to the conventional DH microscopes.
