论文标题
使用全基因组表达汇编来研究微生物
Using genome-wide expression compendia to study microorganisms
论文作者
论文摘要
基因表达纲要是从收集的数据中组装的基因表达实验的异质集合。样品中广泛多样化的实验条件和遗传背景为获得系统级别的理解，对影响表型的转录响应的理解创造了巨大的机会。实验设计的种类对于研究微生物尤其重要，在该微生物中，转录响应整合了许多信号，并在株中表现出可塑性，包括对可用营养物质的反应以及存在哪些微生物的响应。高通量测量技术的进步使得为许多微生物构建纲要是可行的。在这篇综述中，我们讨论了如何构建和分析这些汇编以揭示转录模式。
A gene expression compendium is a heterogeneous collection of gene expression experiments assembled from data collected for diverse purposes. The widely varied experimental conditions and genetic backgrounds across samples creates a tremendous opportunity for gaining a systems level understanding of the transcriptional responses that influence phenotypes. Variety in experimental design is particularly important for studying microbes, where the transcriptional responses integrate many signals and demonstrate plasticity across strains including response to what nutrients are available and what microbes are present. Advances in high-throughput measurement technology have made it feasible to construct compendia for many microbes. In this review we discuss how these compendia are constructed and analyzed to reveal transcriptional patterns.
