论文标题
从形态上鉴定出的小鼠上丘神经元的体内全细胞记录
In vivo whole-cell recording from morphologically identified mouse superior colliculus neurons
论文作者
论文摘要
当生物细胞蛋白标记后的形态表征结合使用时，体内全细胞记录是研究细胞型识别神经元种群中阈值突触加工的强大技术。在这里，我们提供了一个逐步的程序，用于在啮齿动物大脑中主要的视觉处理区域的氨基甲酸酯 - 麻醉小鼠的上丘中执行全细胞记录。描述了两种类型的视觉刺激方法。尽管我们专注于上丘神经元，但该方案适用于其他大脑区域。
In vivo whole-cell recording when combined with morphological characterization after biocytin labeling is a powerful technique to study subthreshold synaptic processing in cell-type-identified neuronal populations. Here, we provide a step-by-step procedure for performing whole-cell recordings in the superior colliculus of urethane-anesthetized mice, a major visual processing region in the rodent brain. Two types of visual stimulation methods are described. While we focus on superior colliculus neurons, this protocol is applicable to other brain areas.
