论文标题
量化确定NMDA受体对诱发和自发突触传播的电流的独立激活的约束
Quantifying constraints determining independent activation on NMDA receptors mediated currents from evoked and spontaneous synaptic transmission at an individual synapse
论文作者
论文摘要
突触通过称为突触前和突触后末端之间融合的化学过程对神经传播作用。突触前末端释放神经递质是响应动作电位或自发独立于突触前活性而释放的。但是，目前尚不清楚诱发和自发的神经传递的机制，该神经传递会激活突触后末端。为了解决这个问题，我们研究了使用数学模拟自发和诱发神经转移的可能性。我们的目的是解决可能决定对诱发和自发性谷氨酸分子释放的N-甲基-D-脱甲酸（NMDA）受体介导的电流的独立激活的生物物理约束。为了确定自发性和诱发的谷氨酸释放之间的空间关系，我们考虑了定量因素，例如突触的大小，扩散迁移率的不均匀性，突触裂解的几何形状以及神经递质的释放速率。仿真结果表明，由于突触尺寸较小，并且如果裂缝空间在外围区域比中心区域更具凝聚力，则具有从中心和边缘释放的两个信号的串扰很高的可能性。当突触尺寸更大时，裂缝空间在中心区域比外部区域更亲和力，如果融合的几何形状的空间较窄，那么这些空间会产生更多独立性的机会，即从中心和边缘释放的两种电流模式。计算出的结果与现有的实验发现非常匹配，并用作进一步探索的路线图，以识别诱发和自发版本的独立性。
A synapse acts on neural transmission through a chemical process called synapses fusion between pre-synaptic and post-synaptic terminals. Presynaptic terminals release neurotransmitters either in response to action potential or spontaneously independent of presynaptic activity. However, it is still unclear the mechanism of evoked and spontaneous neuro-transmission that activate on postsynaptic terminals. To address this question, we examined the possibility that spontaneous and evoked neurotransmissions using mathematical simulations. We aimed to address the biophysical constraints that may determine independent activation on N-methyl-D-asparate (NMDA) receptor mediated currents in response to evoked and spontaneous glutamate molecules releases. In order to identify the spatial relation between spontaneous and evoked glutamate release, we considered quantitative factors, such as size of synapses, inhomogeneity of diffusion mobility, geometry of synaptic cleft, and release rate of neurotransmitter. Simulation results showed that as a synaptic size is smaller and if the cleft space is more cohesive in the peripheral area than the centre area, then there is high possibility of having crosstalk of two signals released from center and edge. When a synaptic size is larger, the cleft space is more affinity in the central area than the external area, and if the geometry of fusion has a narrower space, then those produce more chances of independence of two modes of currents released from center and edge. The computed results match well with existing experimental findings and serve as a road map for further exploration to identify independence of evoked and spontaneous releases.
