论文标题
使用台式极端紫外线成像来可视化微生物的超结构
Visualizing the ultra-structure of microorganisms using table-top extreme ultraviolet imaging
论文作者
论文摘要
桌面极端紫外线（EUV）显微镜为生物样品的无标签研究提供了独特的机会。在这里，我们证明了两个干燥的未染色模型标本的ptychographic EUV成像：真菌（曲霉菌）和细菌（Escherichia coli）细胞的生殖，处于13.5 nm波长。我们发现，迄今为止，EUV光谱区域还没有得到太多关注生物成像，它为鉴定细胞内特征提供了足够的穿透深度。通过实施与位置相关的Ptychography方法，我们证明了通过红外照明启用的毫米平方的视野，并结合了Sub-60 nm的空间分辨率，并通过EUV照明在某些感兴趣的区域上实现了。在13.5 nm波长处的强元对比度可以鉴定样品内部的纳米级材料组成。我们的工作将推进并促进EUV成像应用，并在生命科学中进一步实现可能性。
Table-top extreme ultraviolet (EUV) microscopy offers unique opportunities for label-free investigation of biological samples. Here, we demonstrate ptychographic EUV imaging of two dried, unstained model specimens: germlings of a fungus (Aspergillus nidulans), and bacteria (Escherichia coli) cells at 13.5 nm wavelength. We find that the EUV spectral region, which to date has not received much attention for biological imaging, offers sufficient penetration depths for the identification of intracellular features. By implementing a position-correlated ptychography approach, we demonstrate a millimeter-squared field of view enabled by infrared illumination combined with sub-60 nm spatial resolution achieved with EUV illumination on selected regions of interest. The strong element contrast at 13.5 nm wavelength enables the identification of the nanoscale material composition inside the specimens. Our work will advance and facilitate EUV imaging applications and enable further possibilities in life science.
