论文标题
使用MRI对神经元进行体内标记和定量成像
In vivo labeling and quantitative imaging of neurons using MRI
论文作者
论文摘要
哺乳动物的大脑是一个复杂的器官，包含数十亿个神经元。这些神经元形成各种神经回路，以控制感知，认知，情感和行为。发展体内神经元标记和成像技术对于研究神经回路的结构和功能至关重要。体内技术可以提供真正的生理信息，而实体方法无法提供。在这项研究中，我们描述了使用MRI进行体内神经元标记和定量的新策略。为了证明这种新方法的能力，我们使用了神经性病毒将OATP1A1基因传递到靶神经回路。 OATP1A1蛋白在神经元膜上表达，可以增加特定MRI对比剂（GD-EOB-DTPA）的摄取。通过使用T1加权图像进行观察，将MRI上的神经元标记为“点亮”。我们进一步使用基于动态对比度增强的方法来获取可提供体内标记神经元的定量信息的度量。
Mammalian brain is a complex organ that contains billions of neurons. These neurons form various neural circuits that control the perception, cognition, emotion and behavior. Developing in vivo neuronal labeling and imaging techniques is crucial for studying the structure and function of neural circuits. In vivo techniques can provide true physiological information that cannot be provided by ex vivo methods. In this study, we describe a new strategy for in vivo neuronal labeling and quantification using MRI. To demonstrate the ability of this new method, we used neurotropic virus to deliver oatp1a1 gene to the target neural circuit. OATP1A1 protein is expressed on the neuronal membrane and can increase the uptake of a specific MRI contrast agent (Gd-EOB-DTPA). By using T1-weighted images for observation, labeled neurons "light up" on MRI. We further use a dynamic-contrast-enhancement based method to obtain measures that provide quantitative information of labeled neurons in vivo.
