ICS65.020.20 B05 DB1408 山西省运城市地方标准 DB1408/T045—2023 苹果脱毒组培苗繁育技术规程 2023-07-15发布 2023-10-15实施 运城市市场监督管理局 发布 DB1408/T045—2023 目次 前言......................................................................................................................................................................II 1范围...................................................................................................................................................................1 2规范性引用文件...............................................................................................................................................1 3术语和定义.......................................................................................................................................................1 3.1脱毒............................................................................................................................................................1 3.2叶原基........................................................................................................................................................1 3.3茎尖............................................................................................................................................................1 3.4茎尖分生组织............................................................................................................................................1 3.5离体培养....................................................................................................................................................1 3.6外植体........................................................................................................................................................2 3.7无菌苗........................................................................................................................................................2 4脱毒材料的选取...............................................................................................................................................2 4.1植株初选....................................................................................................................................................2 4.2材料选择....................................................................................................................................................2 5脱毒与培养.......................................................................................................................................................2 5.1外植体消毒................................................................................................................................................2 5.2离体培养无菌苗........................................................................................................................................2 5.3茎尖剥离与接种........................................................................................................................................3 5.4茎尖培养....................................................................................................................................................3 6病毒检测...........................................................................................................................................................3 7组培苗扩繁.......................................................................................................................................................3 7.1培养基制备................................................................................................................................................3 7.2灭菌............................................................................................................................................................3 7.3扩繁............................................................................................................................................................4 7.4瓶苗培养....................................................................