ICS65.020.20 CCSB05 3717 菏泽市地方标准 DB3717/T23—2024 植物种苗组培技术规程 Technicalcodeofplantmicropropagation 2024-06-28发布 2024-07-28实施 菏泽市市场监督管理局  发布 DB3717/T23—2024 I目次 前言........................................................................................................................................................................II 1范围...................................................................................................................................................................1 2规范性引用文件..............................................................................................................................................1 3术语和定义......................................................................................................................................................1 4培养基的配制..................................................................................................................................................1 4.1母液的配制...............................................................................................................................................1 4.2植物接种培养基.......................................................................................................................................1 5植物材料的选择与外植体灭菌......................................................................................................................2 5.1植物组培苗外植体母本圃种植...............................................................................................................2 5.2植物材料选择时间...................................................................................................................................2 5.3植物材料部位的确定和选择...................................................................................................................2 5.4仪器和植物外植体材料的灭菌...............................................................................................................2 5.5仪器及培养基的最少消毒时间...............................................................................................................2 5.6仪器和培养基与外植体消毒...................................................................................................................2 5.7植物外植体的无菌操作...........................................................................................................................3 6组织培养苗试管培养......................................................................................................................................3 6.1愈伤组织诱导分化培养...........................................................................................................................3 6.2增殖继代培养...........................................................................................................................................3 6.3植物组织生根培养...................................................................................................................................3 6.4组织苗炼苗移栽.......................................................................................................................................3 6.5移栽穴盘苗的管理...................................................................................................................................3 7包装与运输......................................................................................................................................................3 7.1包装...........................................................................................................................................................3 7.2运输...........................................................................................................................................................4 附录A（规范性）培养基母液配制................................................................................................................5 附录B（规范性）不同植物品种常用培养基配法........................................................................................6 附录C（规范性）生物调节物质母液的配制...........................................................