ISO INTERNATIONAL 22579 STANDARD IDF 241 First edition 2020-09 Infant formula and adult nutritionals Determination of fructans High performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) after enzymatic treatment Preparations pour nourrissons et produits nutritionnels pour adultesDosagedesfructanesChromatographieéchangeuse d'anions haute performance couplée a la détection par amperométrie pulsee (CEAHP-DAP) apres traitement enzymatique Referencenumbers IS022579:2020(E) IDF 241:2020(E) @IS0andIDF2020 IS0 22579:2020(E) IDF 241:2020(E) COPYRIGHTPROTECTEDDOCUMENT ISOandIDF 2020 All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting below or IsO's member body in the country of the requester: ISO copyright office International Dairy Federation CP 40i : Ch. de Blandonnet 8 Silver Building · Bd Auguste Reyers 70/B CH-1214 Vernier, Geneva B-1030 Brussels Phone:+41227490111 Phone: +32 2 325 67 40 Fax: +32 2 325 67 41 Email: [email protected] Email: [email protected] Website: www.iso.org Website: www.fil-idf.org Published in Switzerland ii ISOand IDF2020-Allrightsreserved IS022579:2020(E) IDF 241:2020(E) Contents Page Foreword iv 1 Scope .1 2 Normative references. .1 3 Terms and definitions .1 Principle .2 4 5 Chemicals and reagents .2 2 5.1 List of chemicals and reagents 5.2 Preparationofreagents 3 5.3 Preparation of mobile phases using column A (6.13.1) or equivalent. 5 5.4 Preparation of mobile phases using columns B (6.13.2) or equivalent 5 5.5 Preparation of standard solutions 6 6 Apparatus .6 7 Procedure .8 7.1 Sample preparation 8 7.1.1 Powdered or concentrated products on a ready-to feed (RTF) basis and powderproductsinhomogeneousatthesubgramlevel .8 7.1.2 Reconstituted products as prepared in Z.1.1 or products sold as RTF .8 7.1.3 Homogeneouspowderedproductswithoutpriorreconstitution 8 7.1.4 Dilution. 8 7.1.5 Hydrolysisof sucroseand α-glucans 8 7.1.6 Carrez clarification (optional, use in case of difficulties passing sample throughSPE) 8 7.1.7 Removalofmonosaccharides 9 7.1.8 Hydrolysis of fructans 9 7.2 Chromatographic conditions using column A (6.13.1) 9 7.3 Chromatographic conditionsusingcolumns B (6.13.2) 10 7.4 System suitability test 11 7.5 Calibration .11 8 Calculation .11 9 Reporting. .13 Precision data 10 .13 10.1 General .13 10.2 Repeatability .13 10.3 Reproducibility ..13 11 .14 Test report Annex A (informative)Example chromatogramsand calibration curves. .15 AnnexB(informative)Precisiondata .18 AnnexC (informative)Checktestenzymemixtureof sucrase,β-amylase,pullulanaseand .19 maltase. Bibliography .22 IS0 and IDF 2020 - All rights reserved iii